| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins
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| País: |
Brazil
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| Título: |
PRODUCTION OF AN EFFECTIVE ANTI-Bothrops-TETANUS MIXED HYPERIMMUNE SERUM OF EQUINE ORIGIN
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| Autores: |
GUIDOLIN,R.
STEPHANO,M. A.
MORAIS,J. F.
SAKAGUTI,E. H.
PRADO,S. M. A.
FRATELLI,F.
VANCETTO,M. D. C.
HIGASHI,H. G.
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| Data: |
1998-01-01
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| Ano: |
1998
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| Palavras-chave: |
Antivenoms
Immunization
Bothrops
Tetanus
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| Resumo: |
The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animals had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by enzymatic digestion and ammonium sulphate precipitation. These experiments lead us to conclude that Bothrops envenomation therapy can be successfully performed using anti-Bothrops-Tetanus serum also serving as Tetanus prophylaxis. Anti-Crotalus-Tetanus serum can also be produced, although it is not of medical interest as Crotalus envenomation rarely results in local necrotizing lesions.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100007
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| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
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| Relação: |
10.1590/S0104-79301998000100007
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| Formato: |
text/html
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| Fonte: |
Journal of Venomous Animals and Toxins v.4 n.1 1998
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| Direitos: |
info:eu-repo/semantics/openAccess
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